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Anti-PHGDH Antibody (7J468)
😃Good
Catalog No. TMAY-02516
Alias SERA, phosphoglycerate dehydrogenase, PHGDHD, PGDH, PGD, PGAD, PDG, NLS1, NLS, HEL-S-113, 3-PGDH, 3PGDH
Anti-PHGDH Antibody (7J468) is a Mouse antibody targeting PHGDH. Anti-PHGDH Antibody (7J468) can be used in FCM,ICC/IF.
Anti-PHGDH Antibody (7J468)
Anti-PHGDH Antibody (7J468)
😃Good
Catalog No. TMAY-02516Alias SERA, phosphoglycerate dehydrogenase, PHGDHD, PGDH, PGD, PGAD, PDG, NLS1, NLS, HEL-S-113, 3-PGDH, 3PGDH
Anti-PHGDH Antibody (7J468) is a Mouse antibody targeting PHGDH. Anti-PHGDH Antibody (7J468) can be used in FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: FCM, ICC/IF Reactivity: Human |
| Alias | SERA, phosphoglycerate dehydrogenase, PHGDHD, PGDH, PGD, PGAD, PDG, NLS1, NLS, HEL-S-113, 3-PGDH, 3PGDH |
| Ig Type | Mouse IgG2b |
| Clone | 7J468 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human PHGDH in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human PHGDH monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-mouse IgG secondary antibody (red). Positive staining was localized to cytoplasm. 2. Flow cytometric analysis of Human PHGDH expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human PHGDH, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | FCM,ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PGDH / PHGDH (rh PGDH / PHGDH; TMPY-02801; O43175; Met1-Phe533). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | PHGDH is a member of the D-isomer specific 2-hydroxyacid dehydrogenase family. This new family consists of D-isomer-stereospecific enzymes. The conserved residues in this family appear to be the residues involved in the substrate binding and the catalytic reaction, and thus to be targets for site-directed mutagenesis. A number of NAD-dependent 2-hydroxyacid dehydrogenases which seem to be specific for the D-isomer of their substrate have been shown to be functionally and structurally related. PHGDH catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. Overexpression of PHGDH may cause certain breast cancers. Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency which is characterized by congenital microcephaly, psychomotor retardation, and seizures. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human PGDH / PHGDH protein (TMPY-02801) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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