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Anti-PHGDH Antibody (7J468)

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Catalog No. TMAY-02516
Alias SERA, phosphoglycerate dehydrogenase, PHGDHD, PGDH, PGD, PGAD, PDG, NLS1, NLS, HEL-S-113, 3-PGDH, 3PGDH

Anti-PHGDH Antibody (7J468) is a Mouse antibody targeting PHGDH. Anti-PHGDH Antibody (7J468) can be used in FCM,ICC/IF.

Anti-PHGDH Antibody (7J468)

Anti-PHGDH Antibody (7J468)

😃Good
Catalog No. TMAY-02516Alias SERA, phosphoglycerate dehydrogenase, PHGDHD, PGDH, PGD, PGAD, PDG, NLS1, NLS, HEL-S-113, 3-PGDH, 3PGDH
Anti-PHGDH Antibody (7J468) is a Mouse antibody targeting PHGDH. Anti-PHGDH Antibody (7J468) can be used in FCM,ICC/IF.
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100 μL$199 7-10 days
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Product Introduction

Bioactivity
Description
Anti-PHGDH Antibody (7J468) is a Mouse antibody targeting PHGDH. Anti-PHGDH Antibody (7J468) can be used in FCM,ICC/IF.
AliasSERA, phosphoglycerate dehydrogenase, PHGDHD, PGDH, PGD, PGAD, PDG, NLS1, NLS, HEL-S-113, 3-PGDH, 3PGDH
Ig Type
Monoclonal Mouse IgG2b
Clone
7J468
Reactivity
Human
Verified Activity
1. Immunofluorescence staining of Human PHGDH in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human PHGDH monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-mouse IgG secondary antibody (red). Positive staining was localized to cytoplasm.

2. Flow cytometric analysis of Human PHGDH expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human PHGDH, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Application
FCM,ICC/IF
Recommended Dose
ICC-IF: 1:20-1:100; FCM: 1:25-1:100
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PGDH / PHGDH (rh PGDH / PHGDH; TMPY-02801; O43175; Met1-Phe533). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundPHGDH is a member of the D-isomer specific 2-hydroxyacid dehydrogenase family. This new family consists of D-isomer-stereospecific enzymes. The conserved residues in this family appear to be the residues involved in the substrate binding and the catalytic reaction, and thus to be targets for site-directed mutagenesis. A number of NAD-dependent 2-hydroxyacid dehydrogenases which seem to be specific for the D-isomer of their substrate have been shown to be functionally and structurally related. PHGDH catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. Overexpression of PHGDH may cause certain breast cancers. Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency which is characterized by congenital microcephaly, psychomotor retardation, and seizures.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human PGDH / PHGDH protein (TMPY-02801)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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