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Anti-PFKM Polyclonal Antibody
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Catalog No. TMAB-01374
Anti-PFKM Polyclonal Antibody is a Rabbit antibody targeting PFKM. Anti-PFKM Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-PFKM Polyclonal Antibody
Anti-PFKM Polyclonal Antibody
😃Good
Catalog No. TMAB-01374
Anti-PFKM Polyclonal Antibody is a Rabbit antibody targeting PFKM. Anti-PFKM Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse,Rat (predicted:Rabbit,Cow,Pig,Chicken) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Rabbit,Cow,Pig,Chicken) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PFKM PFK1) Polyclonal Antibody, Unconjugated (TMAB-01374) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PFKM PFK1) Polyclonal Antibody, Unconjugated (TMAB-01374) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Sample: Lane 1: Human HeLa cell lysates Lane 2: Human HUVEC cell lysates Lane 3: Human U251 cell lysates Primary: Anti-PFKM/PFK1 (TMAB-01374) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 86 kDa Observed band size: 85 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009] |
| Immunogen | KLH conjugated synthetic peptide: human 6 Phosphofructokinase Muscle Type |
| Antigen Species | Human |
| Gene ID | 5213 |
| Uniprot ID | |
| Biology Area | Metabolism of carbohydrates,Carbohydrate metabolism,Energy Metabolism,Energy Metabolism |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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