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Anti-PFKM Polyclonal Antibody (Synonyms: Phosphohexokinase, Phosphofructokinase 1, Phosphofructo-1-kinase isoz...)
Catalog No. TMAB-01374 Copy Product Info
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Anti-PFKM Polyclonal Antibody is a Rabbit antibody targeting PFKM. Anti-PFKM Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
Anti-PFKM Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-01374
Synonyms Phosphohexokinase, Phosphofructokinase 1, Phosphofructo-1-kinase isozyme A, PFKX, PFKM, 6-phosphofructokinase, muscle type
Anti-PFKM Polyclonal Antibody is a Rabbit antibody targeting PFKM. Anti-PFKM Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
Anti-PFKM Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-PFKM Polyclonal Antibody is a Rabbit antibody targeting PFKM. Anti-PFKM Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB. |
| Synonyms | Phosphohexokinase, Phosphofructokinase 1, Phosphofructo-1-kinase isozyme A, PFKX, PFKM, 6-phosphofructokinase, muscle type |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Rabbit,Cow,Pig,Chicken) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PFKM PFK1) Polyclonal Antibody, Unconjugated (TMAB-01374) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PFKM PFK1) Polyclonal Antibody, Unconjugated (TMAB-01374) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Sample: Lane 1: Human HeLa cell lysates Lane 2: Human HUVEC cell lysates Lane 3: Human U251 cell lysates Primary: Anti-PFKM/PFK1 (TMAB-01374) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 86 kDa Observed band size: 85 kDa    |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009] |
| Immunogen | KLH conjugated synthetic peptide: human 6 Phosphofructokinase Muscle Type |
| Antigen Species | Human |
| Gene Name | PFKM |
| Gene ID | |
| Protein Name | ATP-dependent 6-phosphofructokinase, muscle type |
| Uniprot ID | |
| Biology Area | Metabolism of carbohydrates,Carbohydrate metabolism,Energy Metabolism,Energy Metabolism |
| Function | Catalyzes the third step of glycolysis, the phosphorylation of fructose-6-phosphate (F6P) by ATP to generate fructose-1,6-bisphosphate (FBP) and ADP. |
| Molecular Weight | Theoretical: 86 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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