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Anti-PABPN1 Antibody (3P321)
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Catalog No. TMAC-03046
Alias Polyadenylate-binding protein 2, Polyadenylate-binding nuclear protein 1, Poly(A)-binding protein II, Poly(A)-binding protein 2, PABPN 1, PABP-2, PABP2, PABII, PAB2, Nuclear poly(A)-binding protein 1
Anti-PABPN1 Antibody (3P321) is a Rabbit antibody targeting PABPN1. Anti-PABPN1 Antibody (3P321) can be used in WB,ICC/IF,IHC,FCM,IP.
Anti-PABPN1 Antibody (3P321)
Anti-PABPN1 Antibody (3P321)
😃Good
Catalog No. TMAC-03046Alias Polyadenylate-binding protein 2, Polyadenylate-binding nuclear protein 1, Poly(A)-binding protein II, Poly(A)-binding protein 2, PABPN 1, PABP-2, PABP2, PABII, PAB2, Nuclear poly(A)-binding protein 1
Anti-PABPN1 Antibody (3P321) is a Rabbit antibody targeting PABPN1. Anti-PABPN1 Antibody (3P321) can be used in WB,ICC/IF,IHC,FCM,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Chemical Properties
| Description | Anti-PABPN1 Antibody (3P321) is a Rabbit antibody targeting PABPN1. Anti-PABPN1 Antibody (3P321) can be used in WB,ICC/IF,IHC,FCM,IP. |
| Synonyms | Polyadenylate-binding protein 2, Polyadenylate-binding nuclear protein 1, Poly(A)-binding protein II, Poly(A)-binding protein 2, PABPN 1, PABP-2, PABP2, PABII, PAB2, Nuclear poly(A)-binding protein 1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Western blot analysis of PABPN1 on MCF-7 cells lysates using anti-PABPN1 antibody at 1/500 dilution. 2. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PABPN1 antibody. Counter stained with hematoxylin. 3. ICC staining PABPN1 in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining PABPN1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of Hela cells with PABPN1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC/IF: 1:50-200; IP: 1:10-50; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 49 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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