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Anti-p53 Antibody (4S402)

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Catalog No. TMAC-02991

Anti-p53 Antibody (4S402) is a Rabbit antibody targeting p53. Anti-p53 Antibody (4S402) can be used in WB,ICC/IF,IHC,IP.

Anti-p53 Antibody (4S402)

Anti-p53 Antibody (4S402)

😃Good
Catalog No. TMAC-02991
Anti-p53 Antibody (4S402) is a Rabbit antibody targeting p53. Anti-p53 Antibody (4S402) can be used in WB,ICC/IF,IHC,IP.
Pack SizePriceAvailabilityQuantity
50 μL$2977-10 days
100 μL$4977-10 days
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Product Introduction

Bioactivity
Description
Anti-p53 Antibody (4S402) is a Rabbit antibody targeting p53. Anti-p53 Antibody (4S402) can be used in WB,ICC/IF,IHC,IP.
Ig Type
IgG
Clone
4S402
Reactivity
Human
Verified Activity
1. Western blot analysis of p53 on 293 cells lysates using anti-p53 antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-p53 antibody. Counter stained with hematoxylin.
3. ICC staining p53 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining p53 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining p53 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Recommended Dose
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research Backgroundp53, a DNA-binding, oligomerization domain- and transcription activation domain containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 53 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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