Anti-NUDT21 Antibody (9E640) is a Mouse antibody targeting NUDT21. Anti-NUDT21 Antibody (9E640) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG1, Kappa

9E640

Human,Mouse

1. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: room temperature 2 h
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HeLa, 3T3, A431, N2a
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 27 kDa
Observed MW: 27 kDa
2. Cell line: HepG2
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for PTM-6012
3. Tissue: Mouse brain
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: sP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-09693
4. Tissue: Human brain
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-09693

WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500

Monoclonal

NUDT21

Cleavage and polyadenylation specificity factor subunit 5

Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export.