Anti-NLRP3 Polyclonal Antibody 4 is a Rabbit antibody targeting NLRP3. Anti-NLRP3 Polyclonal Antibody 4 can be used in FCM.

IgG

Mouse (predicted:Human,Rat)

Blank control (black line): Raw264.7. Primary Antibody (green line): Rabbit Anti-NALP3/CIAS1 antibody (TMAB-01246)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human Cryopyrin

Human

NLRP3

NACHT, LRR and PYD domains-containing protein 3

TNF,Inflammatory mediators,Macrophage / Inflamm.,NFkB Pathway

May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.