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Anti-NLRP3 Polyclonal Antibody 4
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Catalog No. TMAB-01246
Anti-NLRP3 Polyclonal Antibody 4 is a Rabbit antibody targeting NLRP3. Anti-NLRP3 Polyclonal Antibody 4 can be used in FCM.
Anti-NLRP3 Polyclonal Antibody 4
Anti-NLRP3 Polyclonal Antibody 4
😃Good
Catalog No. TMAB-01246
Anti-NLRP3 Polyclonal Antibody 4 is a Rabbit antibody targeting NLRP3. Anti-NLRP3 Polyclonal Antibody 4 can be used in FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: FCM Reactivity: Mouse (predicted:Human,Rat) |
| Ig Type | IgG |
| Reactivity | Mouse (predicted:Human,Rat) |
| Verified Activity | Blank control (black line): Raw264.7. Primary Antibody (green line): Rabbit Anti-NALP3/CIAS1 antibody (TMAB-01246) Dilution: 1 μg/Test; Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5 μg/Test. Isotype control (orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
| Application | FCM |
| Recommended Dose | FCM: 1ug/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Subcellular Location : Cytoplasm. |
| Immunogen | KLH conjugated synthetic peptide: human Cryopyrin |
| Antigen Species | Human |
| Gene ID | 114548 |
| Uniprot ID | |
| Biology Area | TNF,Inflammatory mediators,Macrophage / Inflamm.,NFkB Pathway |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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