Anti-NKG2D/CD314 Polyclonal Antibody is a Rabbit antibody targeting NKG2D/CD314. Anti-NKG2D/CD314 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat)

1. Blank control (blue line): Mouse thymus cells (blue).
Primary Antibody (green line): Rabbit Anti-NKG2D antibody (TMAB-01240)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 70% methanol (Overnight at 4°C). Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Blank control: SW480. Primary Antibody (green line): Rabbit Anti-NKG2D antibody (TMAB-01240)
Dilution: 2 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Sample: Lung (Mouse) Lysate at 40 μg
Primary: Anti-NKG2D (TMAB-01240) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kDa
Observed band size: 26 kDa

WB,FCM

WB: 1:500-2000; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: rat NKG2D

Rat