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Anti-NFATC4 Polyclonal Antibody

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Catalog No. TMAB-01225

Anti-NFATC4 Polyclonal Antibody is a Rabbit antibody targeting NFATC4. Anti-NFATC4 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-NFATC4 Polyclonal Antibody

Anti-NFATC4 Polyclonal Antibody

😃Good
Catalog No. TMAB-01225
Anti-NFATC4 Polyclonal Antibody is a Rabbit antibody targeting NFATC4. Anti-NFATC4 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$373 7-10 days
200 μL$527 7-10 days
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Product Introduction

Bioactivity
Description
Anti-NFATC4 Polyclonal Antibody is a Rabbit antibody targeting NFATC4. Anti-NFATC4 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Reactivity
Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,Sheep)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NFATC4) Polyclonal Antibody, Unconjugated (TMAB-01225) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NFATC4) Polyclonal Antibody, Unconjugated (TMAB-01225) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
IHC-P,IHC-Fr,IF
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:50-200
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe product of this gene is a member of the nuclearfactors of activated T cells DNA-binding transcription complex.This complex consists of at least two components: a preexistingcytosolic component that translocates to the nucleus upon T cellreceptor (TCR) stimulation and an inducible nuclear component.Other members of this family of nuclear factors of activated Tcells also participate in the formation of this complex. Theproduct of this gene plays a role in the inducible expression ofcytokine genes in T cells, especially in the induction of the IL-2and IL-4. Alternatively spliced transcript variants encodingdifferent isoforms have been noted for this gene. [provided byRefSeq, Oct 2008].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human NF-ATc4
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
NFATs,Other factors,Non-CD,NFATS
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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