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Anti-Neprilysin/CD10 Antibody (8D161)

😃Good
Catalog No. TMAY-00146
Alias SFE, NEP, membrane metallo-endopeptidase, CD10, CALLA

Anti-Neprilysin/CD10 Antibody (8D161) is a Rabbit antibody targeting Neprilysin/CD10. Anti-Neprilysin/CD10 Antibody (8D161) can be used in ICC/IF.

Anti-Neprilysin/CD10 Antibody (8D161)

Anti-Neprilysin/CD10 Antibody (8D161)

😃Good
Catalog No. TMAY-00146Alias SFE, NEP, membrane metallo-endopeptidase, CD10, CALLA
Anti-Neprilysin/CD10 Antibody (8D161) is a Rabbit antibody targeting Neprilysin/CD10. Anti-Neprilysin/CD10 Antibody (8D161) can be used in ICC/IF.
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100 μL$268 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Rabbit Monoclonal

Application: ICC/IF

Reactivity: Human
AliasSFE, NEP, membrane metallo-endopeptidase, CD10, CALLA
Ig Type
Rabbit IgG
Clone
8D161
Reactivity
Human
Verified Activity
Immunofluorescence staining of MME in MCF7 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human MME monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cell membrane.
Application
ICC/IF
Recommended Dose
ICC-IF: 1:20-1:100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human CD10/Neprilysin/MME.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundThe cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alters the behavior of the cell. Some CD proteins do not take part in the cell signal process but have other functions such as cell adhesion. The cluster of differentiation 10 (CD10), also known as Neprilysin and neutral endopeptidase, is a member of the CD system. CD10 is a zinc-dependent metalloprotease enzyme that had the function to degrade some small secreted peptides such as the amyloid beta-peptide. It exists as a membrane-bound protein and has a high concentration in kidney and lung tissues. Mutations in the CD10 gene can induce the familial forms of Alzheimer's disease, providing strong evidence for the protein's association with the Alzheimer's disease process. CD10 is also associated with other biochemical processes.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: center region of the Human CD10/Neprilysin/MME
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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