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Anti-NDUFS3 Antibody (6M78)
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Catalog No. TMAC-02764
Alias NDUFS 3, NADH-ubiquinone oxidoreductase 30 kDa subunit, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, EC 1.6.99.3, EC 1.6.5.3, Complex I-30kD, CI-30kD
Anti-NDUFS3 Antibody (6M78) is a Rabbit antibody targeting NDUFS3. Anti-NDUFS3 Antibody (6M78) can be used in WB,IHC,ICC,IF,IP.
Anti-NDUFS3 Antibody (6M78)
Anti-NDUFS3 Antibody (6M78)
😃Good
Catalog No. TMAC-02764Alias NDUFS 3, NADH-ubiquinone oxidoreductase 30 kDa subunit, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, EC 1.6.99.3, EC 1.6.5.3, Complex I-30kD, CI-30kD
Anti-NDUFS3 Antibody (6M78) is a Rabbit antibody targeting NDUFS3. Anti-NDUFS3 Antibody (6M78) can be used in WB,IHC,ICC,IF,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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Chemical Properties
| Description | Anti-NDUFS3 Antibody (6M78) is a Rabbit antibody targeting NDUFS3. Anti-NDUFS3 Antibody (6M78) can be used in WB,IHC,ICC,IF,IP. |
| Synonyms | NDUFS 3, NADH-ubiquinone oxidoreductase 30 kDa subunit, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, EC 1.6.99.3, EC 1.6.5.3, Complex I-30kD, CI-30kD |
| Ig Type | IgG |
| Clone | 6M78 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of NDUFS3 on HepG2 cell using anti-NDUFS3 antibody at 1/500 dilution. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using anti-NDUFS3 receptor 2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney cancer tissues using anti-NDUFS3 receptor 2 antibody. Counter stained with hematoxylin. 4. ICC staining NDUFS3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining NDUFS3 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:500; IHC: 1:50; ICC: 1:50; IP: 1:10-50 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | NDUFS3 (NADH dehydrogenase ubiquinone iron-sulfur protein 3) is one of about 45 subunits comprising complex I of the oxidative phosphorylation electron transport chain. The multisubunit NADH: ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of the mitochondria. NDUFS3 is the last subunit of the seven subunits that make up the core of complex I. Through use of chaotropic agents, complex I can be separated into three different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. The IP fraction includes NDUFS1-7. NDUFS3 contains a highly conserved casein kinase II phosphorylation site. Mutations in the NDUFS3 gene may cause optic atrophy, Leigh syndrome and complex I deficiency. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 25 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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