Anti-NDUFA9 Polyclonal Antibody is a Rabbit antibody targeting NDUFA9. Anti-NDUFA9 Polyclonal Antibody can be used in WB,FCM.

IgG

Mouse (predicted:Human,Rat,Dog,Pig,Cow,Horse,Rabbit)

1. Sample: Kidney (Mouse) Lysate at 40 μg
Primary: Anti-NDUFA9 (TMAB-09308) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
2. Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-NDUFA9/AF647 Conjugated antibody (TMAB-09308-AF647)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-AF647.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human NDUFA9

Human

NDUFA9

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.