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Anti-Mucin-16/MUC16 Antibody (8O689)
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Catalog No. TMAB-01185
Anti-Mucin-16/MUC16 Antibody (8O689) is a Mouse antibody targeting Mucin-16/MUC16. Anti-Mucin-16/MUC16 Antibody (8O689) can be used in WB,ICC/IF,FCM,IHC-P,IHC-Fr,IF.
Anti-Mucin-16/MUC16 Antibody (8O689)
Anti-Mucin-16/MUC16 Antibody (8O689)
😃Good
Catalog No. TMAB-01185
Anti-Mucin-16/MUC16 Antibody (8O689) is a Mouse antibody targeting Mucin-16/MUC16. Anti-Mucin-16/MUC16 Antibody (8O689) can be used in WB,ICC/IF,FCM,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: WB,ICC/IF,FCM,IHC-P,IHC-Fr,IF Reactivity: Human |
| Ig Type | IgG |
| Clone | 8O689 |
| Reactivity | Human |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Human Ovarian mucinous Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC16 Monoclonal Antibody, Unconjugated (TMAB-01185) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 2. (Negative control) paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC16 Monoclonal Antibody, Unconjugated (TMAB-01185) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC16 Monoclonal Antibody, Unconjugated (TMAB-01185) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC16 Monoclonal Antibody, Unconjugated (TMAB-01185) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Endometrial; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC16 Monoclonal Antibody, Unconjugated (TMAB-01185) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. 25 μg total protein per Lane of various lysates probed with MUC16 monoclonal antibody, unconjugated (TMAB-01185) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 7. 4% Paraformaldehyde-fixed OVCAR-3 (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (MUC16) monoclonal Antibody, unconjugated (TMAB-01185) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control. 8. The OVCAR-3 (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Mouse Anti-MUC16 antibody (TMAB-01185): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488: 1 μg/test. Isotype Control (orange): Mouse IgG. Blank control (black): PBS. |
| Application | WB,ICC/IF,FCM,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1μg/Test; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein. Secreted, extracellular space. Note=May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | MUC16 (CA125) is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is overexpressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller “subunits” being described by some investigators. |
| Immunogen | Recombinant Protein: human MUC16 Protein |
| Antigen Species | Human |
| Gene ID | 94025 |
| Uniprot ID | |
| Biology Area | MUC,Tumor biomarkers,Tumor antigens,MUC |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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