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Anti-MSX1 Polyclonal Antibody is a Rabbit antibody targeting MSX1. Anti-MSX1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-MSX1 Polyclonal Antibody is a Rabbit antibody targeting MSX1. Anti-MSX1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
Ig Type | IgG |
Reactivity | Human,Mouse (predicted:Rat,Dog,Cow) |
Verified Activity | 1. Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-MSX1 Polyclonal Antibody, Unconjugated (TMAB-01172) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Positive control: Hela cells Concebtration: 5 μg/10^6 cells Incubation conditions: Avoid light , 30 minutes on the ice. 3. Sample: Utreus (Mouse) Lysate at 40 μg Hela Cell (Human) Lysate at 40 μg Siha Cell (Human) Lysate at 40 μg A673 Cell (Human) Lysate at 40 μg Primary: Anti-MSX1 (TMAB-01172) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kDa Observed band size: 31 kDa |
Application | WB,IHC-P,IHC-Fr,IF,FCM |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:50-200; FCM: 5μg /Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Nucleus |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | Acts as a transcriptional repressor. May play a role in limb-pattern formation. Acts in cranofacial development and specifically in odontogenesis. Expression in the developing nail bed mesenchyme is important for nail plate thickness and integrity. |
Immunogen | KLH conjugated synthetic peptide: human MSX1 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Nervous system development,Bone,HOX,Transcription Factors,Neural Stem Cell marker,Neurogenesis,Intracellular |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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