Anti-MSX1 Polyclonal Antibody is a Rabbit antibody targeting MSX1. Anti-MSX1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse (predicted:Rat,Dog,Cow)

1. Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-MSX1 Polyclonal Antibody, Unconjugated (TMAB-01172) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Positive control: Hela cells
Concebtration: 5 μg/10^6 cells
Incubation conditions: Avoid light , 30 minutes on the ice.
3. Sample:
Utreus (Mouse) Lysate at 40 μg
Hela Cell (Human) Lysate at 40 μg
Siha Cell (Human) Lysate at 40 μg
A673 Cell (Human) Lysate at 40 μg
Primary: Anti-MSX1 (TMAB-01172) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa

WB,IHC-P,IHC-Fr,IF,FCM

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:50-200; FCM: 5μg /Test

Polyclonal

KLH conjugated synthetic peptide: human MSX1

Human

Nervous system development,Bone,HOX,Transcription Factors,Neural Stem Cell marker,Neurogenesis,Intracellular