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Anti-LGR-5 Polyclonal Antibody 3
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Catalog No. TMAB-01065
Anti-LGR-5 Polyclonal Antibody 3 is a Rabbit antibody targeting LGR-5. Anti-LGR-5 Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-LGR-5 Polyclonal Antibody 3
Anti-LGR-5 Polyclonal Antibody 3
😃Good
Catalog No. TMAB-01065
Anti-LGR-5 Polyclonal Antibody 3 is a Rabbit antibody targeting LGR-5. Anti-LGR-5 Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF,FCM Reactivity: Human,Mouse,Rat |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Sample: Brain (Mouse) Lysate at 40 μg Brain (Rat) Lysate at 40 μg Primary: Anti-LGR5 (TMAB-01065) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 98 kDa Observed band size: 100 kDa 2. Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 μg Lane 2: Stomach (Mouse) Lysate at 40 μg Lane 3: Colon (Mouse) Lysate at 40 μg Primary: Anti-GPR49/LGR5 (TMAB-01065) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100 kDa Observed band size: 105 kDa 3. Paraformaldehyde-fixed, paraffin embedded (mouse placenta tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GPR49) Polyclonal Antibody, Unconjugated (TMAB-01065) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GPR49) Polyclonal Antibody, Unconjugated (TMAB-01065) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 5. Blank control (blue line): U251 (fixed with pre-warmed 4% paraformaldehyde for 30 min at 37°C) Primary Antibody (green line): Rabbit Anti-GPR49 antibody (TMAB-01065), Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1 μg/test. |
| Application | WB,IHC-P,IHC-Fr,IF,FCM |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Multi-pass membrane protein. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] |
| Immunogen | KLH conjugated synthetic peptide: human GPR49/LGR5 |
| Antigen Species | Human |
| Gene ID | 8549 |
| Uniprot ID | |
| Biology Area | GPCR,GPCR,Surface Molecules |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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