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Anti-Latent TGF-beta 2 Polyclonal Antibody
😃Good
Catalog No. TMAB-01049
Anti-Latent TGF-beta 2 Polyclonal Antibody is a Rabbit antibody targeting Latent TGF-beta 2. Anti-Latent TGF-beta 2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Latent TGF-beta 2 Polyclonal Antibody
Anti-Latent TGF-beta 2 Polyclonal Antibody
😃Good
Catalog No. TMAB-01049
Anti-Latent TGF-beta 2 Polyclonal Antibody is a Rabbit antibody targeting Latent TGF-beta 2. Anti-Latent TGF-beta 2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse (predicted:Rat,Rabbit,Cow) |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Rabbit,Cow) |
| Verified Activity | 1. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-TGF-beta-2 Polyclonal Antibody, Unconjugated (TMAB-01049) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Sample: Kidney (Mouse) lysate at 40 μg Primary: Anti-TGF beta 2 Propeptide (TMAB-01049) at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 12/45 kDa Observed band size: 50 kDa 3. Sample: MCF-7 (Human) cell Lysate at 40 μg Primary: Anti-TGF beta 2 Propeptide (TMAB-01049) at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 12/45 kDa Observed band size: 48 kDa 4. Sample: Lane 1: Mouse Heart tissue lysates Lane 2: Mouse Lung tissue lysates Primary: Anti-TGF beta 2 Propeptide (TMAB-01049) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12/45 kDa Observed band size: 48 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Transforming growth factor beta s (TGF beta s) were originally discovered due to their ability to promote anchorage-independent growth of rat NRK fibroblasts in the presence of TGF Alpha. It is now realized that TGF beta s mediate many cell-cell interactions that occur during embryonic development. Three TGF beta s have been identified in mammals. TGF beta 1, TGF beta 2 and TGF beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecules. Biologically active TGF beta requires dimerization of the monomers (usually homodimers) and release of the latent peptide portion. Overall, the mature region of the TGF beta 3 protein has approximately 80% identity to the mature region of both TGF beta 1 and TGF beta 2. However, the NH2 terminals or precursor regions of their molecules share only 27% sequence identity. |
| Immunogen | KLH conjugated synthetic peptide: human TGF-beta 2 |
| Antigen Species | Human |
| Gene ID | 7042 |
| Uniprot ID | |
| Biology Area | Response to hypoxia,TGF,Angiogenic growth factors,Hypoxia,TGF,Secreted |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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