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Anti-ITGAE Polyclonal Antibody
😃Good
Catalog No. TMAB-00993
Anti-ITGAE Polyclonal Antibody is a Rabbit antibody targeting ITGAE. Anti-ITGAE Polyclonal Antibody can be used in WB,FCM.
Anti-ITGAE Polyclonal Antibody
Anti-ITGAE Polyclonal Antibody
😃Good
Catalog No. TMAB-00993
Anti-ITGAE Polyclonal Antibody is a Rabbit antibody targeting ITGAE. Anti-ITGAE Polyclonal Antibody can be used in WB,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,FCM Reactivity: Human,Mouse (predicted:Rat,Dog,Rabbit) |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog,Rabbit) |
| Verified Activity | 1. Sample: LympHnode (Mouse) Lysate at 40 μg Primary: Anti-Integrin alpha E2 (TMAB-00993) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110/128 kDa Observed band size: 128 kDa 2. Sample: U937 (Human) Cell Lysate at 30 μg Raji (Human) Cell Lysate at 30 μg Primary: Anti-Integrin alpha E2 (TMAB-00993) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110/128 kDa Observed band size: 110 kDa 3. Molt-4 cells were fixed with 4% PFA for 10 min at room temperature,permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD103 Antibody (TMAB-00993) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange). |
| Application | WB,FCM |
| Recommended Dose | WB: 1:500-2000; FCM: 1ug/test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, this protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. This protein is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human Integrin alpha-E heavy chain |
| Antigen Species | Human |
| Gene ID | 3682 |
| Uniprot ID | |
| Biology Area | Regulatory T Cells,Adhesion,Alpha,T Lymphocytic Lineage |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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