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Anti-Intrinsic Factor Antibody (1E668)
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Catalog No. TMAY-02597
Alias TCN3, INF, IFMH, IF, gastric intrinsic factor (vitamin B synthesis)
Anti-Intrinsic Factor Antibody (1E668) is a Rabbit antibody targeting Intrinsic Factor. Anti-Intrinsic Factor Antibody (1E668) can be used in FCM,ICC/IF.
Anti-Intrinsic Factor Antibody (1E668)
Anti-Intrinsic Factor Antibody (1E668)
😃Good
Catalog No. TMAY-02597Alias TCN3, INF, IFMH, IF, gastric intrinsic factor (vitamin B synthesis)
Anti-Intrinsic Factor Antibody (1E668) is a Rabbit antibody targeting Intrinsic Factor. Anti-Intrinsic Factor Antibody (1E668) can be used in FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $269 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: FCM, ICC/IF Reactivity: Human |
| Alias | TCN3, INF, IFMH, IF, gastric intrinsic factor (vitamin B synthesis) |
| Ig Type | Rabbit IgG |
| Clone | 1E668 |
| Reactivity | Human |
| Verified Activity | 1. Flow cytometric analysis of Human GIF expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human GIF, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 2. Immunofluorescence staining of GIF in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human GIF monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm. |
| Application | FCM,ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human Gastric intrinsic factor / GIF (rh Gastric intrinsic factor / GIF; TMPY-03221; P27352-1; Met1-Tyr417). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Gastric intrinsic factor, also known as GIF, belongs to the of the cobalamin transport protein family. It is a glycoprotein produced by the parietal cells of the stomach. Gastric intrinsic factor plays a key role in the absorption of vitamin B12 on in the small intestine. Vitamin B12 bounds to haptocorrin after entry into the stomach. The resulting complex enters the duodenum, where pancreatic enzymes digest haptocorrin. In the less acidic environment of the small intestine, B12 can then bind to gastric intrinsic factor. This new complex travels to the ileum, where special epithelial cells endocytose them. Inside the cell, B12 dissociates once again and binds to another protein, transcobalamin II. The new complex can exit the epithelial cells to enter the liver. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human Gastric intrinsic factor / GIF Protein (TMPY-03221) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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