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Anti-Integrin alpha 1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00978
Anti-Integrin alpha 1 Polyclonal Antibody is a Rabbit antibody targeting Integrin alpha 1. Anti-Integrin alpha 1 Polyclonal Antibody can be used in WB,FCM.
Anti-Integrin alpha 1 Polyclonal Antibody
Anti-Integrin alpha 1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00978
Anti-Integrin alpha 1 Polyclonal Antibody is a Rabbit antibody targeting Integrin alpha 1. Anti-Integrin alpha 1 Polyclonal Antibody can be used in WB,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,FCM Reactivity: Human,Rat (predicted:Mouse,Cow,Horse) |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Cow,Horse) |
| Verified Activity | 1. Blank control: RSC96 cells (blue). Primary Antibody: Rabbit Anti-Integrin alpha 1 antibody (TMAB-00978), Dilution: 5 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange),used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. 2. Blank control: U937 (blue). Primary Antibody: Rabbit Anti-Integrin alpha 1 antibody (TMAB-00978), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (TMAB-00978, 1 μg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. 3. Sample: Lane 1: Hela (Human) Cell Lysate at 30 μg Lane 2: 293T (Human) Cell Lysate at 30 μg Lane 3: U87MG (Human) Cell Lysate at 30 μg Lane 4: HepG2 (Human) Cell Lysate at 30 μg Lane 5: A549 (Human) Cell Lysate at 30 μg Primary: Anti-Integrin alpha 1 (TMAB-00978) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 150 kDa Observed band size: 160 kDa |
| Application | WB,FCM |
| Recommended Dose | WB: 1:500-2000; FCM: 1μg /test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around twenty two different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N terminal extracellular domain that binds the ECM ligand and a small C terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function. Alpha 1 integrin along with alpha 2, alpha L and alpha M has a unique inserted domain. Integrin alpha 1 is a receptor for laminin and collagen. The alpha1 subunit is also known as CD49a. CD49a associates with CD29 (beta 1 integrin), to form an alpha1 beta1 heterodimer, identified as the rat homologue to VLA1, which is involved in cellular adhesion to laminin and collagen. |
| Immunogen | KLH conjugated synthetic peptide: human Integrin alpha 1 |
| Antigen Species | Human |
| Gene ID | 3672 |
| Uniprot ID | |
| Biology Area | Basal Lamina,Alpha,Surface Molecules |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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