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Anti-IL-25/IL-17E Polyclonal Antibody is a Rabbit antibody targeting IL-25/IL-17E. Anti-IL-25/IL-17E Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Anti-IL-25/IL-17E Polyclonal Antibody is a Rabbit antibody targeting IL-25/IL-17E. Anti-IL-25/IL-17E Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Alias | interleukin-25, Interleukin-17E, IL-25, IL-17E, IL17E |
Ig Type | IgG |
Reactivity | Human,Mouse (predicted:Rat,Cow,Horse,Rabbit,Sheep) |
Verified Activity | 1. Protein: spleen (mouse) lysate at 30 μg; Primary: rabbit Anti-IL17E (TMAB-00957) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit Igg (secondary antibody) at 1: 5000; Predicted band size: 17 kDa Observed band size: 17 kDa 2. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-IL17E Polyclonal Antibody, Unconjugated (TMAB-00957) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Secreted. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq]. |
Immunogen | KLH conjugated synthetic peptide: human IL17E |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Interleukins |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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