Anti-IL-17C Polyclonal Antibody is a Rabbit antibody targeting IL-17C. Anti-IL-17C Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Rat (predicted:Mouse,Dog,Cow,Horse,Rabbit)

1. Sample:
Lane1: Lung (Rat) Lysate at 30 μg
Lane2: Pancreas (Rat) Lysate at 30 μg
Primary: Anti-IL-17C (TMAB-00947) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit Igg (secondary antibody) at 1: 3000 dilution;
Predicted band size: 20 kDa
Observed band size: 22 kDa
2. Tissue/cell: Human colon cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-IL17C Polyclonal Antibody, Unconjugated (TMAB-00947) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IL17C) Polyclonal Antibody, Unconjugated (TMAB-00947) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human IL-17C

Human

IL17C

Interleukin-17C

Regulatory T Cells,Allergens,Autoimmune,Interleukins

Stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1.