Anti-IDH1 Antibody (6M983) is a Mouse antibody targeting IDH1. Anti-IDH1 Antibody (6M983) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG1, Kappa

6M983

Human,Rat

1. Tissue: Human kidney
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-07493
2. Tissue: Rat stomach
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-07493
3. Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: room temperature 2 h
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: HeLa, 2: SH-SY5Y
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 47 kDa
Observed MW: 47 kDa
4. Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-07493

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500

Monoclonal

IDH1

Isocitrate dehydrogenase [NADP] cytoplasmic