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Anti-HSP20 Antibody (5R128) is a Rabbit antibody targeting HSP20. Anti-HSP20 Antibody (5R128) can be used in WB,IHC,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $298 | 7-10 days | |
100 μL | $498 | 7-10 days |
Description | Anti-HSP20 Antibody (5R128) is a Rabbit antibody targeting HSP20. Anti-HSP20 Antibody (5R128) can be used in WB,IHC,FCM. |
Synonyms | HSPB6_HUMAN, HspB6, Hsp 20, HEL55, Heat-shock 27-KD protein 6, Heat shock protein, 20-KD, Heat shock protein beta-6, Heat shock protein beta 6, Heat shock protein alpha crystallin related B6, Heat shock 20 kDa-like protein p20, Heat shock 20 kDa like protein p20, FLJ32389, epididymis luminal protein 55 |
Ig Type | IgG |
Clone | 5R128 |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of Hsp20 on different lysates using anti-Hsp20 antibody at 1/1,000 dilution. Positive control: Lane 1: Human skeletal muscle, Lane 2: Mouse skeletal muscle. 2. Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Hsp20 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Hsp20 antibody. Counter stained with hematoxylin. 4. Flow cytometric analysis of Hela cells with Hsp20 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Application | |
Recommended Dose | WB: 1:1000-2000; IHC: 1:50-500; FCM: 1:50-100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent poly-peptide chains across cellular membranes and regulation of protein folding. Heat shock proteins (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins and the HSP 110 family. The low molecular weight family includes HSP 10, HSP 20, HSP 27 (Heme Oxygenase 1), HSP 32 and HSP 40. HSP 20 occurs in two complex sizes, dimers and multimers. It is related to stress proteins and occurs most abundantly in skeletal muscle and heart. HSP 20 is considerably shorter at the C-terminus and less polar than other small heat shock proteins. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 17 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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