Anti-HSD17B3 Polyclonal Antibody is a Rabbit antibody targeting HSD17B3. Anti-HSD17B3 Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P, WB.

IgG

Mouse,Rat (predicted:Human,Pig,Cow,Horse,Rabbit)

1. Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HSD17B3) Polyclonal Antibody, Unconjugated (TMAB-00890) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-HSD17B3 Polyclonal Antibody, Unconjugated (TMAB-00890) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Brain (Mouse) Lysate at 40 μg
Testis (Mouse) Lysate at 40 μg
Primary: Anti-HSD17B3 (TMAB-00890) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (secondary antibody) at 1/5000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human HSD17B3

Human

HSD17B3

Testosterone 17-beta-dehydrogenase 3

Hormone biosynthesis,Metabolism of lipids and lipoproteins,Oxidative Stress,Hormone biosynthesis,Lipid metabolism,Oxidative stress,Cancer,Hormones

Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH.