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Anti-HSD17B3 Polyclonal Antibody

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Catalog No. TMAB-00890

Anti-HSD17B3 Polyclonal Antibody is a Rabbit antibody targeting HSD17B3. Anti-HSD17B3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-HSD17B3 Polyclonal Antibody

Anti-HSD17B3 Polyclonal Antibody

😃Good
Catalog No. TMAB-00890
Anti-HSD17B3 Polyclonal Antibody is a Rabbit antibody targeting HSD17B3. Anti-HSD17B3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$372 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Anti-HSD17B3 Polyclonal Antibody is a Rabbit antibody targeting HSD17B3. Anti-HSD17B3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Pig,Cow,Horse,Rabbit)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HSD17B3) Polyclonal Antibody, Unconjugated (TMAB-00890) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-HSD17B3 Polyclonal Antibody, Unconjugated (TMAB-00890) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Brain (Mouse) Lysate at 40 μg
Testis (Mouse) Lysate at 40 μg
Primary: Anti-HSD17B3 (TMAB-00890) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (secondary antibody) at 1/5000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationTestis.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundHSD17B3 is involved in the reduction of androstenedione to testosterone. It is expressed predominantly in the testis. Deficiency in HSD17B3 are the cause of male pseudohermaphrodism with gynecomastia (MPH).
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human HSD17B3
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Hormone biosynthesis,Metabolism of lipids and lipoproteins,Oxidative Stress,Hormone biosynthesis,Lipid metabolism,Oxidative stress,Cancer,Hormones
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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