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Anti-HRH1 Polyclonal Antibody
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Catalog No. TMAB-00889
Alias HRH1, Histamine H1 receptor, HH1R, H1-R, H1R
Anti-HRH1 Polyclonal Antibody is a Rabbit antibody targeting HRH1. Anti-HRH1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-HRH1 Polyclonal Antibody
Anti-HRH1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00889Alias HRH1, Histamine H1 receptor, HH1R, H1-R, H1R
Anti-HRH1 Polyclonal Antibody is a Rabbit antibody targeting HRH1. Anti-HRH1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-HRH1 Polyclonal Antibody is a Rabbit antibody targeting HRH1. Anti-HRH1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | HRH1, Histamine H1 receptor, HH1R, H1-R, H1R |
| Reactivity | Human (predicted:Mouse,Rat,Dog,Pig,Cow) |
| Verified Activity | 1. Sample: Lane 1: U87MG (Human) Cell Lysate at 30 μg Lane 2: U251 (Human) Cell Lysate at 30 μg Lane 3: Huvec (Human) Cell Lysate at 30 μg Lane 4: Hela (Human) Cell Lysate at 30 μg Primary: Anti-HRH1 (TMAB-00889) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kDa Observed band size: 56 kDa 2. Sample: LOVO (Human) Cell Lysate at 30 μg Primary: Anti-HRH1 (TMAB-00889) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa 3. Paraformaldehyde-fixed, paraffin embedded (Human ovarian cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HRH1) Polyclonal Antibody, Unconjugated (TMAB-00889) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Multi-pass membrane protein. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene was thought to be intronless until recently. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human HRH1 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Allergens,Macrophage / Inflamm.,Hormones,GPCR |
| Molecular Weight | Theoretical: 54 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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