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Anti-HRAS Polyclonal Antibody

Catalog No. TMAB-07283 Copy Product Info
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Anti-HRAS Polyclonal Antibody is a Rabbit antibody targeting HRAS. Anti-HRAS Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-HRAS Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-07283

Anti-HRAS Polyclonal Antibody is a Rabbit antibody targeting HRAS. Anti-HRAS Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-HRAS Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2227-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-HRAS Polyclonal Antibody is a Rabbit antibody targeting HRAS. Anti-HRAS Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Rat (predicted:Human,Mouse,Pig)
Verified Activity
1. Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-HRAS/Ras/Ras p21 Polyclonal Antibody, Unconjugated (TMAB-07283) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-HRAS/Ras/Ras p21 Polyclonal Antibody, Unconjugated (TMAB-07283) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Note=Shuttles between the plasma membrane and the Golgi apparatus.
Tissue SpecificityWidely expressed.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundN-RAS (neuroblastoma RAS viral oncogene homolog) analogously to other GTP-binding proteins (such as Translation Elongation Factor EFTu or signal transducing G-Proteins) RAS proteins are involved in signal transduction pathways, ras gene family is part of the ras superfamily including the mammalian RAS, RAL, RAC, RHO, RAP, and RAB gene families and the yeast homologs like SEC4 and YPT1 genes; genes encode small monomeric proteins of low molecular mass (20-30 kDa) which share at least 30% homology to RAS proteins.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human H-ras
Antigen Species
Human
Gene Name
NRAS
Gene ID
Protein Name
GTPase NRas
Uniprot ID
Function
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Chemical Properties
Molecular WeightTheoretical: 21 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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