Anti-HMOX1 Antibody (9D115) is a Rabbit antibody targeting HMOX1. Anti-HMOX1 Antibody (9D115) can be used in FCM,ICC/IF,IF,IHC-Fr,IHC-P,WB.

IgG

9D115

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (TMAB-00876) at 1:1000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat thymus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (TMAB-00876) at 1:1000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (TMAB-00876) at 1:1000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (TMAB-00876) at 1:1000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (TMAB-00876) at 1:1000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (mouse thymus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (TMAB-00876) at 1:1000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
7. Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Placenta tissue lysates
Lane 3: Mouse NIH/3T3 cell lysates
Lane 4: Rat Spleen tissue lysates
Lane 5: Rat Liver tissue lysates
Lane 6: Human HeLa cell lysates
Lane 7: Human 293T cell lysates
Lane 8: Human HepG2 cell lysates
Primary: Anti-Heme Oxygenase 1 (TMAB-00876) at 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 30 kDa
8. 25 μg total protein per Lane of various lysates probed with Heme Oxygenase 1/HMOX1 monoclonal antibody, unconjugated (TMAB-00876) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.

WB: 1:2000-20000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500; FCM: 1:50-100

Monoclonal

KLH conjugated synthetic peptide: human Heme Oxygenase 1

Human

HMOX1

Heme oxygenase 1

Response to hypoxia,Inflammatory mediators,Platelets,Endothelium,NFkB pathway,Hypoxia,Cancer,Heart disease,Neurodegenerative disease,Alzheimer's disease,NFkB Pathway

Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.