Anti-HAS2 Polyclonal Antibody is a Rabbit antibody targeting HAS2. Anti-HAS2 Polyclonal Antibody can be used in WB,FCM.

IgG

Human (predicted:Mouse,Rat,Chicken,Pig,Cow,Horse,Rabbit,Sheep)

1. Sample: Huh7 Cell (Human) Lysate at 40 μg
Primary: Anti-HAS2 (TMAB-00830) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (secondary antibody) at 1/5000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
2. Sample:
Lane 1: Human U251 cell Lysates
Lane 2: Human U-87 MG cell Lysates
Lane 3: Human Hela cell Lysates
Primary: Anti-HAS2 (TMAB-00830) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 60 kDa
3. Blank control (black line): Hela.
Primary Antibody (green line): Rabbit Anti-HAS2 antibody (TMAB-00830)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
4. Blank control (black line): Hela.
Primary Antibody (green line): Rabbit Anti-HAS2 antibody (TMAB-00830)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB,FCM

WB: 1:500-2000; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human HAS2/Hyaluronan synthase 2

Human