Anti-GRIA1 Polyclonal Antibody is a Rabbit antibody targeting GRIA1. Anti-GRIA1 Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P, IP, WB.

IgG

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Mouse hippocampal; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GluR1 Polyclonal Antibody, Unconjugated (TMAB-00810) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat hippocampal; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GluR1 Polyclonal Antibody, Unconjugated (TMAB-00810) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GluR1 Polyclonal Antibody, Unconjugated (TMAB-00810) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GluR1 Polyclonal Antibody, Unconjugated (TMAB-00810) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GluR1 Polyclonal Antibody, Unconjugated (TMAB-00810) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
6. (Negative control) paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GluR1 Polyclonal Antibody, Unconjugated (TMAB-00810) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
7. 25 μg total protein per Lane of various lysates probed with GluR1 polyclonal antibody, unconjugated (TMAB-00810) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.

IF=1:100-200; IHC-Fr=1:100-200; IHC-P=1:100-200; IP=1:20-50; WB=1:500-2000

Polyclonal

KLH conjugated synthetic peptide: human GluR1

Human

GRIA1

Glutamate receptor 1

Glutamate Receptors,AMPA / Kainate

Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.