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Anti-GRIA1 Polyclonal Antibody is a Rabbit antibody targeting GRIA1. Anti-GRIA1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-GRIA1 Polyclonal Antibody is a Rabbit antibody targeting GRIA1. Anti-GRIA1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,IP. |
Alias | OTTHUMP00000224243, OTTHUMP00000224242, OTTHUMP00000224241, OTTHUMP00000165781, OTTHUMP00000160643, MGC133252, HBGR1, GRIA1, Gria 1, Glutamate receptor, ionotropic, AMPA 1, Glutamate receptor ionotropic AMPA 1, Glutamate receptor ionotropic, Glutamate receptor 1, GluR-K1, GluRK1, GluR-A, GLURA, GLUR1, GluR K1, GLUR A, GLUR 1, GLUH1, GLUH 1, AMPA selective glutamate receptor 1, AMPA 1 |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Mouse hippocampal; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GluR1 Polyclonal Antibody, Unconjugated (TMAB-00810) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Rat hippocampal; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GluR1 Polyclonal Antibody, Unconjugated (TMAB-00810) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GluR1 Polyclonal Antibody, Unconjugated (TMAB-00810) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GluR1 Polyclonal Antibody, Unconjugated (TMAB-00810) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GluR1 Polyclonal Antibody, Unconjugated (TMAB-00810) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. (Negative control) paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GluR1 Polyclonal Antibody, Unconjugated (TMAB-00810) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 7. 25 μg total protein per Lane of various lysates probed with GluR1 polyclonal antibody, unconjugated (TMAB-00810) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. |
Application | WB,IHC-P,IHC-Fr,IF,IP |
Recommended Dose | WB: 1:500-1:2000; IHC-P: 1:100-1:200; IHC-Fr: 1:100-1:200; IF: 1:100-1:200; IP: 1:20-1:50 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, dendrit. Cell projection, dendritic spine. Note=Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. |
Immunogen | KLH conjugated synthetic peptide: human GluR1 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Glutamate Receptors,AMPA / Kainate |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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