Anti-GM-CSFR alpha Polyclonal Antibody is a Rabbit antibody targeting GM-CSFR alpha. Anti-GM-CSFR alpha Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat)

1. Paraformaldehyde-fixed, paraffin embedded (Human Melanocytoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GM-CSFR alpha) Polyclonal Antibody, Unconjugated (TMAB-06597) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-GM-CSFR alpha/CD116 Polyclonal Antibody, Unconjugated (TMAB-06597) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-GM-CSFR alpha/CD116 Polyclonal Antibody, Unconjugated (TMAB-06597) 1: 200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1: 200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml, blue) was used to stain the cell nuclei

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: mouse GM-CSFR alpha

Mouse

CSF2RA

Granulocyte-macrophage colony-stimulating factor receptor subunit alpha

Low affinity receptor for granulocyte-macrophage colony-stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.