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Anti-GHR/Growth Hormone R Polyclonal Antibody is a Rabbit antibody targeting GHR/Growth Hormone R. Anti-GHR/Growth Hormone R Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-GHR/Growth Hormone R Polyclonal Antibody is a Rabbit antibody targeting GHR/Growth Hormone R. Anti-GHR/Growth Hormone R Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Sheep) |
Verified Activity | 1. Blank control: A549 (blue), the cells were fixed with 2% paraformaldehyde (10 min). Isotype Control Antibody: Rabbit Igg (orange); Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA; Primary Antibody Dilution: 5 μL in 100 μL 1X PBS containing 0.5% BSA (green). 2. This image was generously provided by Brandon Menzies, PhD, from The University of Melbourne. Paraffin embedded tammar wallaby (Macropus eugenii) liver labeled with Rabbit Anti-GHR Polyclonal Antibody, Unconjugated (TMAB-00768) at 1:300 followed by conjugation to a secondary antibody and staining. 3. Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-GHR Polyclonal Antibody, Unconjugated (TMAB-00768) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 4. Positive control: A549 cells Concebtration: 1 μg/10^6 cells Incubation conditions: Avoid light , 30 minutes on the ice. 5. Sample: Lane 1: Testis (Mouse) Lysate at 40 μg Lane 2: Cerebrum (Mouse) Lysate at 40 μg Lane 3: Testis (Rat) Lysate at 40 μg Lane 4: Cerebrum (Rat) Lysate at 40 μg Primary: Anti-Growth hormone receptor (TMAB-00768) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130/110 kDa Observed band size: 130 kDa |
Application | WB,IHC-P,IHC-Fr,IF,FCM |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cell membrane; Single-pass type I membrane protein. Note=On growth hormone binding, GHR is ubiquitinated, internalized, down-regulated and transported into a degradative or non-degradative pathway. Isoform 2: Cell membrane; Single-pass type I membrane protein. Note=Remains fixed to the cell membrane and is not internalized. Growth hormone-binding protein: Secreted. Note=Complexed to a substantial fraction of circulating GH. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Immunogen | KLH conjugated synthetic peptide: human GHR |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Cell differentiation,Growth hormones,Hormones |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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