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Anti-Fibronectin Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00679
Anti-Fibronectin Polyclonal Antibody 2 is a Rabbit antibody targeting Fibronectin. Anti-Fibronectin Polyclonal Antibody 2 can be used in WB,ICC/IF.
Anti-Fibronectin Polyclonal Antibody 2
Anti-Fibronectin Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00679
Anti-Fibronectin Polyclonal Antibody 2 is a Rabbit antibody targeting Fibronectin. Anti-Fibronectin Polyclonal Antibody 2 can be used in WB,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,ICC/IF Reactivity: Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Sample: Lane 1: Kidney (Mouse) Lysate at 40 μg Lane 2: Cerebellum (Rat) Lysate at 40 μg Primary: Anti-Fibronectin/Ugl-Y3 (TMAB-00679) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 263 kDa Observed band size: 263 kDa 2. Tissue/cell: HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Fibronectin/Ugl-Y3) Polyclonal Antibody, Unconjugated (TMAB-00679) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | WB,ICC/IF |
| Recommended Dose | WB: 1:500-2000; ICC/IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human Fibronectin/Ugl-Y3 |
| Antigen Species | Human |
| Gene ID | 2335 |
| Uniprot ID | |
| Biology Area | Extracellular matrix,Extracellular Matrix,Ectoderm,Fibronectin,Ectoderm,Osteogenesis,Extracellular |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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