Anti-Fibronectin Polyclonal Antibody 2 is a Rabbit antibody targeting Fibronectin. Anti-Fibronectin Polyclonal Antibody 2 can be used in ICC/IF,WB.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit)

1. Sample:
Lane 1: Kidney (Mouse) Lysate at 40 μg
Lane 2: Cerebellum (Rat) Lysate at 40 μg
Primary: Anti-Fibronectin/Ugl-Y3 (TMAB-00679) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 263 kDa
Observed band size: 263 kDa
2. Tissue/cell: HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Fibronectin/Ugl-Y3) Polyclonal Antibody, Unconjugated (TMAB-00679) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; ICC/IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Fibronectin/Ugl-Y3

Human

FN1

Fibronectin

Extracellular matrix,Extracellular Matrix,Ectoderm,Fibronectin,Ectoderm,Osteogenesis,Extracellular

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.