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Anti-FCGBP Polyclonal Antibody

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Catalog No. TMAB-00665
Alias IgGFc-binding protein, Human Fc gamma BP, FCGBP, FcgammaBP, Fcgamma-binding protein antigen, FC(GAMMA)BP, Fc fragment of IgG binding protein

Anti-FCGBP Polyclonal Antibody is a Rabbit antibody targeting FCGBP. Anti-FCGBP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-FCGBP Polyclonal Antibody

Anti-FCGBP Polyclonal Antibody

😃Good
Catalog No. TMAB-00665Alias IgGFc-binding protein, Human Fc gamma BP, FCGBP, FcgammaBP, Fcgamma-binding protein antigen, FC(GAMMA)BP, Fc fragment of IgG binding protein
Anti-FCGBP Polyclonal Antibody is a Rabbit antibody targeting FCGBP. Anti-FCGBP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3747-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-FCGBP Polyclonal Antibody is a Rabbit antibody targeting FCGBP. Anti-FCGBP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasIgGFc-binding protein, Human Fc gamma BP, FCGBP, FcgammaBP, Fcgamma-binding protein antigen, FC(GAMMA)BP, Fc fragment of IgG binding protein
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Rabbit)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FCGBP) Polyclonal Antibody, Unconjugated (TMAB-00665) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FCGBP) Polyclonal Antibody, Unconjugated (TMAB-00665) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FCGBP) Polyclonal Antibody, Unconjugated (TMAB-00665) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-FCGBP Polyclonal Antibody, Unconjugated (TMAB-00665) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
5. Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-FCGBP Polyclonal Antibody, Unconjugated (TMAB-00665) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
6. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FCGBP) Polyclonal Antibody, Unconjugated (TMAB-00665) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FCGBP) Polyclonal Antibody, Unconjugated (TMAB-00665) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
8. Positive sample: Paraformaldehyde-fixed, paraffin embedded (rat intestine);
Negative sample: Paraformaldehyde-fixed, paraffin embedded (rat lung);
Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FCGBP) Polyclonal Antibody, Unconjugated (TMAB-00665) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
9. Sample:
Lane 1: Human A549 cell lysates
Lane 2: Human Jurkat cell lysates
Primary: Anti-FCGBP (TMAB-00665) at 1/200 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 569 kDa
Observed band size: 120 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:200-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundFc (Ig constant fragment) receptors ensure protection of the host against foreign antigens, such as microorganisms and pathogens, by removing Ig-coated antigen complexes from circulation. Fc receptors are present on lymphoid and myeloid derivatives, where they mediate endocytosis of Ig-antigen complexes, antibody production in B cells through T cell antigen presentation, cytotoxicity and the release of cytokines and reactive oxygen species. The Fc γ-binding protein (FCGBP) interacts with the Fc portion of IgG and MUC2 to mediate the maintenance of the mucosal structure. FCGBP is a 5,405 amino acid protein that contains twelve TIL (trypsin inhibitory-like) domains and thirteen VWFD domains. It is predominantly expressed in placenta and colon epithelium as well as in thyroid and serum. Patients with various autoimmune diseases seemingly have higher levels of FCGBP protein present in their serum.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human FCGBP
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
IgG,Receptors
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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