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Anti-FCGBP Polyclonal Antibody
😃Good
Catalog No. TMAB-00665
Alias IgGFc-binding protein, Human Fc gamma BP, FCGBP, FcgammaBP, Fcgamma-binding protein antigen, FC(GAMMA)BP, Fc fragment of IgG binding protein
Anti-FCGBP Polyclonal Antibody is a Rabbit antibody targeting FCGBP. Anti-FCGBP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-FCGBP Polyclonal Antibody
Anti-FCGBP Polyclonal Antibody
😃Good
Catalog No. TMAB-00665Alias IgGFc-binding protein, Human Fc gamma BP, FCGBP, FcgammaBP, Fcgamma-binding protein antigen, FC(GAMMA)BP, Fc fragment of IgG binding protein
Anti-FCGBP Polyclonal Antibody is a Rabbit antibody targeting FCGBP. Anti-FCGBP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-FCGBP Polyclonal Antibody is a Rabbit antibody targeting FCGBP. Anti-FCGBP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | IgGFc-binding protein, Human Fc gamma BP, FCGBP, FcgammaBP, Fcgamma-binding protein antigen, FC(GAMMA)BP, Fc fragment of IgG binding protein |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FCGBP) Polyclonal Antibody, Unconjugated (TMAB-00665) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FCGBP) Polyclonal Antibody, Unconjugated (TMAB-00665) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (rat intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FCGBP) Polyclonal Antibody, Unconjugated (TMAB-00665) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-FCGBP Polyclonal Antibody, Unconjugated (TMAB-00665) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 5. Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-FCGBP Polyclonal Antibody, Unconjugated (TMAB-00665) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 6. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FCGBP) Polyclonal Antibody, Unconjugated (TMAB-00665) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded (mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FCGBP) Polyclonal Antibody, Unconjugated (TMAB-00665) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 8. Positive sample: Paraformaldehyde-fixed, paraffin embedded (rat intestine); Negative sample: Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FCGBP) Polyclonal Antibody, Unconjugated (TMAB-00665) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 9. Sample: Lane 1: Human A549 cell lysates Lane 2: Human Jurkat cell lysates Primary: Anti-FCGBP (TMAB-00665) at 1/200 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 569 kDa Observed band size: 120 kDa |
| Application | |
| Recommended Dose | WB: 1:200-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Fc (Ig constant fragment) receptors ensure protection of the host against foreign antigens, such as microorganisms and pathogens, by removing Ig-coated antigen complexes from circulation. Fc receptors are present on lymphoid and myeloid derivatives, where they mediate endocytosis of Ig-antigen complexes, antibody production in B cells through T cell antigen presentation, cytotoxicity and the release of cytokines and reactive oxygen species. The Fc γ-binding protein (FCGBP) interacts with the Fc portion of IgG and MUC2 to mediate the maintenance of the mucosal structure. FCGBP is a 5,405 amino acid protein that contains twelve TIL (trypsin inhibitory-like) domains and thirteen VWFD domains. It is predominantly expressed in placenta and colon epithelium as well as in thyroid and serum. Patients with various autoimmune diseases seemingly have higher levels of FCGBP protein present in their serum. |
| Immunogen | KLH conjugated synthetic peptide: human FCGBP |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | IgG,Receptors |
| Molecular Weight | Theoretical: 569 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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