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Anti-FABP4 Antibody (5W917) is a Mouse antibody targeting FABP4. Anti-FABP4 Antibody (5W917) can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $297 | 7-10 days | |
100 μL | $489 | 7-10 days |
Description | Anti-FABP4 Antibody (5W917) is a Mouse antibody targeting FABP4. Anti-FABP4 Antibody (5W917) can be used in WB,IHC-P,IHC-Fr,IF. |
Alias | HEL-S-104, fatty acid binding protein 4, adipocyte, aP2, ALBP, A-FABP, AFABP |
Clone | 5W917 |
Reactivity | Mouse (predicted:Human) |
Verified Activity | 1. Sample: Lane 1: Mouse Adipose tissue lysates Lane 2: Mouse Breast tissue lysates Lane 3: Mouse Heart tissue lysates Lane 4: Mouse Thymus tissue lysates Primary: Anti-FABP4 (TMAB-00648) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa 2. Paraformaldehyde-fixed, paraffin embedded Mouse Brown Fat; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FABP4 Monoclonal Antibody, Unconjugated (TMAB-00648) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FABP4 Monoclonal Antibody, Unconjugated (TMAB-00648) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FABP4 Monoclonal Antibody, Unconjugated (TMAB-00648) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FABP4 Monoclonal Antibody, Unconjugated (TMAB-00648) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:20-200; IF: 1:100-500 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Subcellular Localization | Cytoplasm. Nucleus. Note=Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export. |
Construction | Hybridoma Monoclonal Antibody |
Purification | Protein G purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | FABP4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq]. |
Immunogen | Recombinant Protein: human FABP4 protein |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Metabolism of lipids and lipoproteins,Binding proteins,Metabolism,Fatty acids,Lipid metabolism,Fatty acid oxidation,Cancer,Lipid metabolism,Adipogenesis |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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