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Anti-EIF3K Antibody (9B286)
😃Good
Catalog No. TMAY-02740
Alias PTD001, PRO1474, PLAC-24, PLAC24, MSTP001, M9, HSPC029, eukaryotic translation initiation factor 3, subunit K, EIF3S12, EIF3-p28, ARG134
Anti-EIF3K Antibody (9B286) is a Mouse antibody targeting EIF3K. Anti-EIF3K Antibody (9B286) can be used in ICC/IF.
Anti-EIF3K Antibody (9B286)
Anti-EIF3K Antibody (9B286)
😃Good
Catalog No. TMAY-02740Alias PTD001, PRO1474, PLAC-24, PLAC24, MSTP001, M9, HSPC029, eukaryotic translation initiation factor 3, subunit K, EIF3S12, EIF3-p28, ARG134
Anti-EIF3K Antibody (9B286) is a Mouse antibody targeting EIF3K. Anti-EIF3K Antibody (9B286) can be used in ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: ICC/IF Reactivity: Human |
| Alias | PTD001, PRO1474, PLAC-24, PLAC24, MSTP001, M9, HSPC029, eukaryotic translation initiation factor 3, subunit K, EIF3S12, EIF3-p28, ARG134 |
| Ig Type | Mouse IgG1 |
| Clone | 9B286 |
| Reactivity | Human |
| Verified Activity | Immunofluorescence staining of Human EIF3K in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human EIF3K monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green). Positive staining was localized to cytoplasm and nucleus. |
| Application | ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EIF3K (rh EIF3K; TMPY-03343; Q9UBQ5; Ala2-Gln218). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | EIF3K is a member of the eIF3 subunit K family. It is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 4S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). It stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. EIF3K is universally expressed in human tissues. It is distributed both in nucleus and cytoplasm. EIF3K is the smallest subunit of eIF3 and it interacts with a number of other subunits of eIF3 and the 4S ribosomal subunit. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human EIF3K Protein (TMPY-03343) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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