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Anti-eIF3E Antibody (6U507)

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Catalog No. TMAB-05503

Anti-eIF3E Antibody (6U507) is a Rabbit antibody targeting eIF3E. Anti-eIF3E Antibody (6U507) can be used in FCM, ICC/IF, WB.

Anti-eIF3E Antibody (6U507)

Anti-eIF3E Antibody (6U507)

Copy Product Info
😃Good
Catalog No. TMAB-05503
Anti-eIF3E Antibody (6U507) is a Rabbit antibody targeting eIF3E. Anti-eIF3E Antibody (6U507) can be used in FCM, ICC/IF, WB.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1517-10 days7-10 days
50 μL$2627-10 days7-10 days
100 μL$4737-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-eIF3E Antibody (6U507) is a Rabbit antibody targeting eIF3E. Anti-eIF3E Antibody (6U507) can be used in FCM, ICC/IF, WB.
Ig Type
IgG
Clone
6U507
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of (1) 293T cell lysate; (2) Jurkat cell lysate. Using eIF3E (TMAB-05503) monoclonal antibody at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The 293T (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-eIF3E antibody (TMAB-05503, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
Application
Recommended Dose
FCM=1:50-100; ICC/IF=1:50-200; WB=1:1000-2000
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis.
Antigen Details
Immunogen
A synthesized peptide: human eIF3E
Antigen Species
Human
Gene Name
EIF3E
Gene ID
Protein Name
Eukaryotic translation initiation factor 3 subunit E
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 52 kDa. Actual: 45 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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