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Anti-EDG1 Polyclonal Antibody is a Rabbit antibody targeting EDG1. Anti-EDG1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-EDG1 Polyclonal Antibody is a Rabbit antibody targeting EDG1. Anti-EDG1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Alias | Sphingosine 1phosphate receptor type 1 S1P1, Sphingosine 1-phosphate receptor Edg-1, Sphingosine 1-phosphate receptor 1, Sphingosine 1 phosphate receptor EDG1, S1PR1, S1P1, S1P receptor Edg-1, S1P receptor 1, G protein coupled sphingolipid receptor, Endothelial differentiation G-protein coupled receptor 1, EDG 1, ECGF1, D1S3362, CHEDG1, CD363 |
Reactivity | Human,Mouse,Rat (predicted:Dog,Horse) |
Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EDG1) Polyclonal Antibody, Unconjugated (TMAB-00588) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EDG1) Polyclonal Antibody, Unconjugated (TMAB-00588) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Sample: HUVEc (Human) Cell Lysate at 30 μg Primary: Anti-EDG1 (TMAB-00588) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kDa Observed band size: 54 kDa 4. Sample: Lane 1: Mouse Cerebellum tissue lysates Lane 2: Mouse Cerebrum tissue lysates Lane 3: Rat Cerebellum tissue lysates Lane 4: Rat Cerebrum tissue lysates Primary: Anti-EDG1 (TMAB-00588) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kDa Observed band size: 50 kDa |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cell membrane; Multi-pass membrane protein. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | Sphingosine-1-phosphate receptor 1 (S1P receptor 1 or S1P1), also known as endothelial differentiation gene 1 (EDG1) is a protein that in humans is encoded by the S1PR1 gene. S1PR1 is a G-protein-coupled receptor which binds the bioactive signaling molecule sphingosine 1-phosphate (S1P). S1PR1 belongs to a sphingosine-1-phosphate receptor subfamily comprising five members (S1PR1-5). S1PR1 was originally identified as an abundant transcript in endothelial cells and it has an important role in regulating endothelial cell cytoskeletal structure, migration, capillary-like network formation and vascular maturation. In addition, S1PR1 signaling is important in the regulation of lymphocyte maturation, migration and trafficking. |
Immunogen | KLH conjugated synthetic peptide: human EDG1/CD363 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Associated Proteins,Endothelial Cell Markers,Associated Proteins,Endothelial,GPCR,Lipid Signaling |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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