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Anti-EDG1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00588
Alias Sphingosine 1phosphate receptor type 1 S1P1, Sphingosine 1-phosphate receptor Edg-1, Sphingosine 1-phosphate receptor 1, Sphingosine 1 phosphate receptor EDG1, S1PR1, S1P1, S1P receptor Edg-1, S1P receptor 1, G protein coupled sphingolipid receptor, Endothelial differentiation G-protein coupled receptor 1, EDG 1, ECGF1, D1S3362, CHEDG1, CD363

Anti-EDG1 Polyclonal Antibody is a Rabbit antibody targeting EDG1. Anti-EDG1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-EDG1 Polyclonal Antibody

Anti-EDG1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00588Alias Sphingosine 1phosphate receptor type 1 S1P1, Sphingosine 1-phosphate receptor Edg-1, Sphingosine 1-phosphate receptor 1, Sphingosine 1 phosphate receptor EDG1, S1PR1, S1P1, S1P receptor Edg-1, S1P receptor 1, G protein coupled sphingolipid receptor, Endothelial differentiation G-protein coupled receptor 1, EDG 1, ECGF1, D1S3362, CHEDG1, CD363
Anti-EDG1 Polyclonal Antibody is a Rabbit antibody targeting EDG1. Anti-EDG1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3737-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-EDG1 Polyclonal Antibody is a Rabbit antibody targeting EDG1. Anti-EDG1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasSphingosine 1phosphate receptor type 1 S1P1, Sphingosine 1-phosphate receptor Edg-1, Sphingosine 1-phosphate receptor 1, Sphingosine 1 phosphate receptor EDG1, S1PR1, S1P1, S1P receptor Edg-1, S1P receptor 1, G protein coupled sphingolipid receptor, Endothelial differentiation G-protein coupled receptor 1, EDG 1, ECGF1, D1S3362, CHEDG1, CD363
Reactivity
Human,Mouse,Rat (predicted:Dog,Horse)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EDG1) Polyclonal Antibody, Unconjugated (TMAB-00588) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EDG1) Polyclonal Antibody, Unconjugated (TMAB-00588) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Sample:
HUVEc (Human) Cell Lysate at 30 μg
Primary: Anti-EDG1 (TMAB-00588) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 54 kDa
4. Sample:
Lane 1: Mouse Cerebellum tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Rat Cerebellum tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Primary: Anti-EDG1 (TMAB-00588) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 50 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Multi-pass membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundSphingosine-1-phosphate receptor 1 (S1P receptor 1 or S1P1), also known as endothelial differentiation gene 1 (EDG1) is a protein that in humans is encoded by the S1PR1 gene. S1PR1 is a G-protein-coupled receptor which binds the bioactive signaling molecule sphingosine 1-phosphate (S1P). S1PR1 belongs to a sphingosine-1-phosphate receptor subfamily comprising five members (S1PR1-5). S1PR1 was originally identified as an abundant transcript in endothelial cells and it has an important role in regulating endothelial cell cytoskeletal structure, migration, capillary-like network formation and vascular maturation. In addition, S1PR1 signaling is important in the regulation of lymphocyte maturation, migration and trafficking.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human EDG1/CD363
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Associated Proteins,Endothelial Cell Markers,Associated Proteins,Endothelial,GPCR,Lipid Signaling
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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