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Anti-DLL4 Polyclonal Antibody
😃Good
Catalog No. TMAB-00554
Anti-DLL4 Polyclonal Antibody is a Rabbit antibody targeting DLL4. Anti-DLL4 Polyclonal Antibody can be used in WB.
Anti-DLL4 Polyclonal Antibody
Anti-DLL4 Polyclonal Antibody
😃Good
Catalog No. TMAB-00554
Anti-DLL4 Polyclonal Antibody is a Rabbit antibody targeting DLL4. Anti-DLL4 Polyclonal Antibody can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-DLL4 Polyclonal Antibody is a Rabbit antibody targeting DLL4. Anti-DLL4 Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse) |
| Verified Activity | 1. Sample: Lane 1: Human K562 cell Lysates Lane 2: Human THP-1 cell Lysates Lane 3: Human 293T cell Lysates Primary: Anti-DLL4 (TMAB-00554) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 75 kDa Observed band size: 75 kDa 2. Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Delta 4 Polyclonal Antibody, Unconjugated (TMAB-00554) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
| Application | WB |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type I membrane protein (Probable). |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The LIN-12/Notch family of transmembrane receptors is believed to play a central role in development by regulating cell fate decisions. Notch proteins have been found to be overexpressed or rearranged in human tumors. Ligands for Notch include Jagged, Jagged-2 and Delta. While blocking the differentiation of progenitor cells into the B-cell lineage, Delta promotes the emergence of a population of cells with T cell/NK-cell characteristics. The protein is a membrane protein expressed in heart, pancreas, brain and muscle during gastrulation and early organogenesis and in adult heart and lung. Delta-4 is a membrane protein that activates Notch-1 and Notch-4. It is expressed in a wide range of adult and fetal tissues, especially in vascular endothelium. |
| Immunogen | KLH conjugated synthetic peptide: human DLL4 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Cell differentiation,Cell Differentiation,Notch Pathway |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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