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Anti-DARPP-32 Antibody (1B13)
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Catalog No. TMAC-01115
Alias Protein phosphatase 1 regulatory subunit 1B, Protein phosphatase 1 regulatory inhibitor subunit 1B, Protein phosphatase 1 regulatory (inhibitor) subunit 1B, PPR1B_HUMAN, PPP1R1B, Neuronal phosphoprotein DARPP 32, IPPD, FLJ20940, Dopamine- and cAMP-regulated neuronal phosphoprotein, Dopamine and cAMP regulated phosphoprotein DARPP32, Dopamine and cAMP regulated phosphoprotein DARPP 32, Dopamine and cAMP regulated phosphoprotein, Dopamine and cAMP regulated neuronal phosphoprotein 32, Dopamine and cAMP regulated neuronal phosphoprotein, DARPP32, DARPP 32
Anti-DARPP-32 Antibody (1B13) is a Rabbit antibody targeting DARPP-32. Anti-DARPP-32 Antibody (1B13) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-DARPP-32 Antibody (1B13)
Anti-DARPP-32 Antibody (1B13)
😃Good
Catalog No. TMAC-01115Alias Protein phosphatase 1 regulatory subunit 1B, Protein phosphatase 1 regulatory inhibitor subunit 1B, Protein phosphatase 1 regulatory (inhibitor) subunit 1B, PPR1B_HUMAN, PPP1R1B, Neuronal phosphoprotein DARPP 32, IPPD, FLJ20940, Dopamine- and cAMP-regulated neuronal phosphoprotein, Dopamine and cAMP regulated phosphoprotein DARPP32, Dopamine and cAMP regulated phosphoprotein DARPP 32, Dopamine and cAMP regulated phosphoprotein, Dopamine and cAMP regulated neuronal phosphoprotein 32, Dopamine and cAMP regulated neuronal phosphoprotein, DARPP32, DARPP 32
Anti-DARPP-32 Antibody (1B13) is a Rabbit antibody targeting DARPP-32. Anti-DARPP-32 Antibody (1B13) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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| Description | Anti-DARPP-32 Antibody (1B13) is a Rabbit antibody targeting DARPP-32. Anti-DARPP-32 Antibody (1B13) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Synonyms | Protein phosphatase 1 regulatory subunit 1B, Protein phosphatase 1 regulatory inhibitor subunit 1B, Protein phosphatase 1 regulatory (inhibitor) subunit 1B, PPR1B_HUMAN, PPP1R1B, Neuronal phosphoprotein DARPP 32, IPPD, FLJ20940, Dopamine- and cAMP-regulated neuronal phosphoprotein, Dopamine and cAMP regulated phosphoprotein DARPP32, Dopamine and cAMP regulated phosphoprotein DARPP 32, Dopamine and cAMP regulated phosphoprotein, Dopamine and cAMP regulated neuronal phosphoprotein 32, Dopamine and cAMP regulated neuronal phosphoprotein, DARPP32, DARPP 32 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of DARPP32 on mouse brain lysates using anti-DARPP32 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DARPP32 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-DARPP32 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-DARPP32 antibody. Counter stained with hematoxylin. 5. ICC staining DARPP32 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining DARPP32 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of Hela cells with DARPP32 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Dopaminergic signaling pathways, which are essential for multiple brain functions, are abnormal in several neurological disorders, such as schizophrenia, Parkinson's disease and drug abuse. DARPP-32 (for dopamine and adenosine 3',5'-monophosphate-regulated phosphoprotein) is abundant in neurons that receive dopaminergic input. Activation of PKA and the consequent phosphorylation of DARPP-32 on threonine occurs in response to dopamine acting upon D1-like receptors. Dopamine interaction with D2-like receptors results in the inhibition of PKA activation, the activation of protein phosphatase 2B and the consequent dephosphorylation of DARPP-32. Neurotransmitters other than dopamine may also be able to stimulate the phosphorylation or dephosphorylation of DARPP-32. Phosphorylated DARPP-32 is a potent inhibitor of PP-1. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 32 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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