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Anti-Cytokeratin 5 Polyclonal Antibody
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Catalog No. TMAB-00532
Anti-Cytokeratin 5 Polyclonal Antibody is a Rabbit antibody targeting Cytokeratin 5. Anti-Cytokeratin 5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Cytokeratin 5 Polyclonal Antibody
Anti-Cytokeratin 5 Polyclonal Antibody
😃Good
Catalog No. TMAB-00532
Anti-Cytokeratin 5 Polyclonal Antibody is a Rabbit antibody targeting Cytokeratin 5. Anti-Cytokeratin 5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat bladder tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (TMAB-00532) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (TMAB-00532) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 3. Sample: Lane 1: Skin (Mouse) Lysate at 40 μg Lane 2: Uterus (Mouse) Lysate at 40 μg Lane 3: Urinary bladder (Mouse) Lysate at 40 μg Lane 4: Esophagus (Mouse) Lysate at 40 μg Lane 5: Skin (Rat) Lysate at 40 μg Lane 6: Uterus (Rat) Lysate at 40 μg Lane 7: Urinary bladder (Rat) Lysate at 40 μg Lane 8: Esophagus (Rat) Lysate at 40 μg Primary: Anti-Cytokeratin 5 (TMAB-00532) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kDa Observed band size: 60 kDa 4. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (TMAB-00532) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinising tissue (ie., skin) and non keratinising cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of 40 to 70 kDa and isoelectric points of 5 to 8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Defects in KRT5 are a cause of epidermolysis bullosa simplex. |
| Immunogen | KLH conjugated synthetic peptide: human CK5 |
| Antigen Species | Human |
| Gene ID | 3852 |
| Uniprot ID | |
| Biology Area | Cytokeratins |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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