Anti-CYCS Polyclonal Antibody is a Rabbit antibody targeting CYCS. Anti-CYCS Polyclonal Antibody can be used in WB.

IgG

Mouse,Rat (predicted:Pig,Cow,Horse)

1. Sample:
Cytochrome C protein at 30 μg
Primary: Anti-Cytochrome C (TMAB-00516) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
2. Blank control: HepG2 (blue). Primary Antibody: Rabbit Anti-Cytochrome C antibody (TMAB-00516,Green); Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde for 10 min at 37°C. Primary antibody (TMAB-00516, 1 μg/1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature.
3. Tissue/cell: SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Cytochrome C) polyclonal Antibody, Unconjugated (TMAB-00516) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
4. Sample:
Lane 1: Heart (Rat) Lysate at 40 μg
Lane 2: Heart (Mouse) Lysate at 40 μg
Primary: Anti-Cytochrome C (TMAB-00516) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 14.4 kDa
Observed band size: 14.4 kDa

WB

WB: 1:500-2000

Polyclonal

KLH conjugated synthetic peptide: human Cytochrome C

Human

Integration of energy metabolism,Cytochrome C,Metabolism,Mitochondrial,Cytochrome C,Cytochromes,Mitochondrial,Energy Metabolism,Integration of energy,Lipases,Apoptosis,Cytochromes,Mitochondrial markers,Oxidative phosphorylation,Energy Metabolism,Mitochondrial