Anti-CYCS Antibody (7Q735) is a Mouse antibody targeting CYCS. Anti-CYCS Antibody (7Q735) can be used in ICC/IF, IF, IHC-Fr, IHC-P, WB.

IgG

7Q735

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (TMAB-00515) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (TMAB-00515) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (TMAB-00515) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
4. SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Cytochrome C) monoclonal Antibody, Unconjugated (TMAB-00515) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
5. Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (ascites of TMAB-00515) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (TMAB-00515) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
7. Sample:
Hcclm3 (Human) Cell Lysate at 30 μg
Primary: Anti-Cytochrome C (TMAB-00515) at 1/100000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
8. Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Mouse Muscle tissue lysates
Lane 3: Mouse Cerebrum tissue lysates
Lane 4: Rat Muscle tissue lysates
Lane 5: Human Jurkat cell lysates
Lane 6: Human HL-60 cell lysates
Lane 7: Human U251 cell lysates
Primary: Anti-Cytochrome C (TMAB-00515) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
9. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (TMAB-00515) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (TMAB-00515) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (TMAB-00515) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (TMAB-00515) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
13. Paraformaldehyde-fixed, paraffin embedded (human Prostate Tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (TMAB-00515) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

ICC/IF=1:100-500; IF=1:200-800; IHC-Fr=1:200-800; IHC-P=1:200-800; WB=1:2000-20000

Monoclonal

Recombinant Protein: human Cytochrome C Protein

Human

CYCS

Cytochrome c

Integration of energy metabolism,Cytochrome C,Metabolism,Mitochondrial,Cytochrome C,Cytochromes,Mitochondrial,Energy Metabolism,Integration of energy,Lipases,Apoptosis,Cytochromes,Mitochondrial markers,Oxidative phosphorylation,Energy Metabolism,Mitochondrial

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.