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Anti-Cyclin B2 Antibody (9U494) (Synonyms: MGC140694, MGC108931, HsT17299, G2/mitotic-specific cyclin-B2, G2...)

Catalog No. TMAC-01035 Copy Product Info
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Anti-Cyclin B2 Antibody (9U494) is a Rabbit antibody targeting Cyclin B2. Anti-Cyclin B2 Antibody (9U494) can be used in ICC/IF,IHC,IP,WB.

Anti-Cyclin B2 Antibody (9U494)

Copy Product Info
🥰Excellent
Catalog No. TMAC-01035
Synonyms MGC140694, MGC108931, HsT17299, G2/mitotic-specific cyclin-B2, G2/mitotic specific cyclin B2, G2 mitotic specific cyclin B2, Cyclin B2, CycB2, CCNB2_HUMAN, ccnb2

Anti-Cyclin B2 Antibody (9U494) is a Rabbit antibody targeting Cyclin B2. Anti-Cyclin B2 Antibody (9U494) can be used in ICC/IF,IHC,IP,WB.

Anti-Cyclin B2 Antibody
(9U494)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2987-10 days7-10 days
100 μL$4987-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Cyclin B2 Antibody (9U494) is a Rabbit antibody targeting Cyclin B2. Anti-Cyclin B2 Antibody (9U494) can be used in ICC/IF,IHC,IP,WB.
SynonymsMGC140694, MGC108931, HsT17299, G2/mitotic-specific cyclin-B2, G2/mitotic specific cyclin B2, G2 mitotic specific cyclin B2, Cyclin B2, CycB2, CCNB2_HUMAN, ccnb2
Ig Type
IgG
Clone
9U494
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of Cyclin B2 on different lysates using anti-Cyclin B2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: K562.
2. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cyclin B2 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Cyclin B2 antibody. Counter stained with hematoxylin.
4. ICC staining Cyclin B2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining Cyclin B2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
Application
Recommended Dose
WB: 1:1000; IHC: 1:50-200; ICC/IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundIn eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M-phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc 1). The Cdc/cyclin enzyme is subject to multiple levels of control of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B enzyme and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex . Evidence has established that B-type cyclins not only act on M-phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B-type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 45 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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