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Anti-Cyclin B1 Antibody (8M905)
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Catalog No. TMAB-04822 Copy Product Info
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Anti-Cyclin B1 Antibody (8M905) is a Mouse antibody targeting Cyclin B1. Anti-Cyclin B1 Antibody (8M905) can be used in IHC-P,IHC-Fr,IF.
Anti-Cyclin B1 Antibody
(8M905)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 µL | $223 | 7-10 days | 7-10 days | |
| 100 µL | $372 | 7-10 days | 7-10 days | |
| 200 µL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-Cyclin B1 Antibody (8M905) is a Mouse antibody targeting Cyclin B1. Anti-Cyclin B1 Antibody (8M905) can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Clone | 8M905 |
| Reactivity | Human (predicted:Mouse,Rat) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Human esophageal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cyclin B1) Monoclonal Antibody, Unconjugated (TMAB-04822 7D1) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Human esophageal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cyclin B1) Monoclonal Antibody, Unconjugated (ascites of TMAB-04822 7D1) at 1: 2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:500-1000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human Cyclin B1 |
| Antigen Species | Human |
| Gene Name | CCNB1 |
| Gene ID | |
| Protein Name | G2/mitotic-specific cyclin-B1 |
| Uniprot ID | |
| Function | Essential for the control of the cell cycle at the G2/M (mitosis) transition. |
| Molecular Weight | Theoretical: 48 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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