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Anti-Cyclin B1 Antibody (8M905)

Catalog No. TMAB-04822 Copy Product Info
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Anti-Cyclin B1 Antibody (8M905) is a Mouse antibody targeting Cyclin B1. Anti-Cyclin B1 Antibody (8M905) can be used in IHC-P,IHC-Fr,IF.

Anti-Cyclin B1 Antibody (8M905)

Copy Product Info
🥰Excellent
Catalog No. TMAB-04822

Anti-Cyclin B1 Antibody (8M905) is a Mouse antibody targeting Cyclin B1. Anti-Cyclin B1 Antibody (8M905) can be used in IHC-P,IHC-Fr,IF.

Anti-Cyclin B1 Antibody
(8M905)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2237-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Cyclin B1 Antibody (8M905) is a Mouse antibody targeting Cyclin B1. Anti-Cyclin B1 Antibody (8M905) can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Clone
8M905
Reactivity
Human (predicted:Mouse,Rat)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Human esophageal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cyclin B1) Monoclonal Antibody, Unconjugated (TMAB-04822 7D1) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Human esophageal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cyclin B1) Monoclonal Antibody, Unconjugated (ascites of TMAB-04822 7D1) at 1: 2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:500-1000
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationCytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome.
ConstructionHybridoma Monoclonal Antibody
PurificationProtein G purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Cyclin B1
Antigen Species
Human
Gene Name
CCNB1
Gene ID
Protein Name
G2/mitotic-specific cyclin-B1
Uniprot ID
Function
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Chemical Properties
Molecular WeightTheoretical: 48 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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