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Anti-Cyclin A2 Antibody (9J817)
😃Good
Catalog No. TMAC-01026
Alias Cyclin-A2, Cyclin-A, Cyclin A2, CCNA2_HUMAN, Ccna2, CCNA, CCN1
Anti-Cyclin A2 Antibody (9J817) is a Rabbit antibody targeting Cyclin A2. Anti-Cyclin A2 Antibody (9J817) can be used in ICC/IF,IHC,WB.
Anti-Cyclin A2 Antibody (9J817)
Anti-Cyclin A2 Antibody (9J817)
😃Good
Catalog No. TMAC-01026Alias Cyclin-A2, Cyclin-A, Cyclin A2, CCNA2_HUMAN, Ccna2, CCNA, CCN1
Anti-Cyclin A2 Antibody (9J817) is a Rabbit antibody targeting Cyclin A2. Anti-Cyclin A2 Antibody (9J817) can be used in ICC/IF,IHC,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Chemical Properties
| Description | Anti-Cyclin A2 Antibody (9J817) is a Rabbit antibody targeting Cyclin A2. Anti-Cyclin A2 Antibody (9J817) can be used in ICC/IF,IHC,WB. |
| Synonyms | Cyclin-A2, Cyclin-A, Cyclin A2, CCNA2_HUMAN, Ccna2, CCNA, CCN1 |
| Ig Type | IgG |
| Clone | 9J817 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of Cyclin A2 on different lysates using anti-Cyclin A2 antibody at 1/1,000 dilution. Positive control: Lane 1: PC12, Lane 2: NIH/3T3. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin. 6. ICC staining Cyclin A2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Cyclin A2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining Cyclin A2 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. The best-characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of maturing promoting factor. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin- dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F and retinoblastoma protein, p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 49 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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