Anti-CREB-1 Polyclonal Antibody 2 is a Mouse antibody targeting CREB-1. Anti-CREB-1 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Sheep)

1. Tissue/cell: Human endometrium tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Mouse Anti-CREB-1 Polyclonal Antibody, Unconjugated (TMAB-04648) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Lane 1: Kidney (Mouse) Lysate at 40 μg
Lane 2: Kidney (Rat) Lysate at 40 μg
Lane 3: Testis (Mouse) Lysate at 40 μg
Lane 4: Testis (Rat) Lysate at 40 μg
Lane 5: Liver (Mouse) Lysate at 40 μg
Primary: Anti-CREB-1 (TMAB-04648) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 45 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human CREB-1

Human

CREB1

Cyclic AMP-responsive element-binding protein 1

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.