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Anti-CRB2 Polyclonal Antibody
😃Good
Catalog No. TMAB-00476
Alias RP11-230L22.2, Crumbs-like protein 2, Crumbs homolog 2(Drosophila), Crumbs homolog 2, CRUM2, Crb2
Anti-CRB2 Polyclonal Antibody is a Rabbit antibody targeting CRB2. Anti-CRB2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-CRB2 Polyclonal Antibody
Anti-CRB2 Polyclonal Antibody
😃Good
Catalog No. TMAB-00476Alias RP11-230L22.2, Crumbs-like protein 2, Crumbs homolog 2(Drosophila), Crumbs homolog 2, CRUM2, Crb2
Anti-CRB2 Polyclonal Antibody is a Rabbit antibody targeting CRB2. Anti-CRB2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CRB2 Polyclonal Antibody is a Rabbit antibody targeting CRB2. Anti-CRB2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | RP11-230L22.2, Crumbs-like protein 2, Crumbs homolog 2(Drosophila), Crumbs homolog 2, CRUM2, Crb2 |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CRB2) Polyclonal Antibody, Unconjugated (TMAB-00476) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Eye (Rat) Lysate at 40 μg Primary: Anti-CRB2 (TMAB-00476) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100/123/131 kDa Observed band size: 135 kDa 3. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-CRB2 (TMAB-00476) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100，123，131 kDa Observed band size: 100,131 kDa 4. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-CRB2 Polyclonal Antibody, Unconjugated (TMAB-00476) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 5. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-CRB2 Polyclonal Antibody, Unconjugated (TMAB-00476) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted and Cell membrane. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | CRB2 is a 1,285 amino acid protein that contains three laminin G-like domains and 15 EGF-like domains and exists as multiple alternatively spliced isoforms that are either secreted or membrane bound. Expressed in kidney and retina, as well as in fetal eye and brain, CRB2 is thought to play a role in the morphogenesis of polarized cells and may be involved in DNA repair. Defects in the gene encoding CRB2 are associated with the pathogenesis of autosomal recessive retinitis pigmentosa (RP) and Leber congenital amaurosis (LCA), the former of which refers to a group of diseases that lead to the degeneration of retinal photoreceptor cells, ultimately resulting in a loss of vision. The gene encoding CRB2 maps to human chromosome 9, which houses over 900 genes and comprises nearly 4% of the human genome. |
| Immunogen | KLH conjugated synthetic peptide: human CRB2 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Other Antibodies |
| Molecular Weight | Theoretical: 100/123/131 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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