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Anti-Complement Factor D/CFD Polyclonal Antibody
😃Good
Catalog No. TMAB-00467
Alias PFD, FACTOR D, EC 3.4.21.46, EC 3.4.21, DF, Complement factor D, CFD, C3 convertase activator, AMBP-1, ADN, Adipsin
Anti-Complement Factor D/CFD Polyclonal Antibody is a Rabbit antibody targeting Complement Factor D/CFD. Anti-Complement Factor D/CFD Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Complement Factor D/CFD Polyclonal Antibody
Anti-Complement Factor D/CFD Polyclonal Antibody
😃Good
Catalog No. TMAB-00467Alias PFD, FACTOR D, EC 3.4.21.46, EC 3.4.21, DF, Complement factor D, CFD, C3 convertase activator, AMBP-1, ADN, Adipsin
Anti-Complement Factor D/CFD Polyclonal Antibody is a Rabbit antibody targeting Complement Factor D/CFD. Anti-Complement Factor D/CFD Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Complement Factor D/CFD Polyclonal Antibody is a Rabbit antibody targeting Complement Factor D/CFD. Anti-Complement Factor D/CFD Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | PFD, FACTOR D, EC 3.4.21.46, EC 3.4.21, DF, Complement factor D, CFD, C3 convertase activator, AMBP-1, ADN, Adipsin |
| Reactivity | Human,Mouse (predicted:Rat) |
| Verified Activity | 1. Protein: A549 (human) cell lysates at 40 μg; spleen disease in mouse at 40 μg; Primary: Anti-Factor D (TMAB-00467) at 1:300; Secondary: HRP conjugated Goat-Anti-Rabbit Igg (secondary antibody) at 1: 5000; ECL excitated the fluorescence; Predicted band size:24 kDa Observed band size:28/75 kDa 2. Tissue/cell: Human lung cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Factor D Polyclonal Antibody, Unconjugated (TMAB-00467) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Adipsin is the mouse homolog of the previously described human complement Factor D, a serine protease, which is now designated human Adipsin. Human Adipsin is highly expressed in and secreted by adipose tissue, and it has also been found in monocytes and macrophages. Rodent Adipsin has only been detected in high levels in adipose tissue. It has been shown that complement factor B, when complexed with activated complement component C3, is cleaved by Adipsin. While low expression of Adipsin has been confirmed in obese mice with hypothalamic defects, this inverse correlation between Adipsin expression and obesity has not been demonstrated in humans. |
| Immunogen | KLH conjugated synthetic peptide: human Factor D/Adipsin |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Alternative Pathway |
| Molecular Weight | Theoretical: 24 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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