Anti-Complement Factor D/CFD Polyclonal Antibody is a Rabbit antibody targeting Complement Factor D/CFD. Anti-Complement Factor D/CFD Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

Human,Mouse (predicted:Rat)

1. Protein:
A549 (human) cell lysates at 40 μg;
spleen disease in mouse at 40 μg;
Primary: Anti-Factor D (TMAB-00467) at 1:300;
Secondary: HRP conjugated Goat-Anti-Rabbit Igg (secondary antibody) at 1: 5000;
ECL excitated the fluorescence;
Predicted band size:24 kDa
Observed band size:28/75 kDa
2. Tissue/cell: Human lung cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Factor D Polyclonal Antibody, Unconjugated (TMAB-00467) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Factor D/Adipsin

Human

CFD

Complement factor D

Alternative Pathway

Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.