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Anti-Collagen IV/COL4A1 Polyclonal Antibody 2
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Catalog No. TMAB-00461
Anti-Collagen IV/COL4A1 Polyclonal Antibody 2 is a Rabbit antibody targeting Collagen IV/COL4A1. Anti-Collagen IV/COL4A1 Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF.
Anti-Collagen IV/COL4A1 Polyclonal Antibody 2
Anti-Collagen IV/COL4A1 Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00461
Anti-Collagen IV/COL4A1 Polyclonal Antibody 2 is a Rabbit antibody targeting Collagen IV/COL4A1. Anti-Collagen IV/COL4A1 Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: IHC-P,IHC-Fr,IF Reactivity: Human |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Collagen IV) Polyclonal Antibody, Unconjugated (TMAB-00461) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Collagen IV) Polyclonal Antibody, Unconjugated (TMAB-00461) at 1:600 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Collagen IV) Polyclonal Antibody, Unconjugated (TMAB-00461) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Collagen IV) Polyclonal Antibody, Unconjugated (TMAB-00461) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Collagen IV) Polyclonal Antibody, Unconjugated (TMAB-00461) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | IHC-P,IHC-Fr,IF |
| Recommended Dose | IHC-P: 1:200-1000; IHC-Fr: 1:200-1000; IF: 1:200-1000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix, basement membrane. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human Collagen alpha-1(IV) chain |
| Antigen Species | Human |
| Gene ID | 1282 |
| Uniprot ID |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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