Anti-Collagen III Antibody (5A23) is a Rabbit antibody targeting Collagen III. Anti-Collagen III Antibody (5A23) can be used in WB,FCM,ICC/IF,IHC-P,IHC-Fr,IF.

IgG

5A23

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Monoclonal Antibody, Unconjugated (TMAB-04524) at 1:500 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Monoclonal Antibody, Unconjugated (TMAB-04524) at 1:500 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Monoclonal Antibody, Unconjugated (TMAB-04524) at 1:500 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Abdominal skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Monoclonal Antibody, Unconjugated (TMAB-04524) at 1:500 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Monoclonal Antibody, Unconjugated (TMAB-04524) at 1:500 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Monoclonal Antibody, Unconjugated (TMAB-04524) at 1:500 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Monoclonal Antibody, Unconjugated (TMAB-04524) at 1:500 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Monoclonal Antibody, Unconjugated (TMAB-04524) at 1:500 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
9. 25 μg total protein per lane of various lysates (see on figure) probed with Collagen III monoclonal antibody, unconjugated (TMAB-04524) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
10. The RD (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-Collagen III antibody (TMAB-04524), Dilution： 1:100; Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
11. 4% Paraformaldehyde-fixed RD (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Collagen III) monoclonal Antibody, unconjugated (TMAB-04524) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

WB: 1:500-2000; FCM: 1:50-100; ICC/IF: 1:50-200; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

Recombinant Protein: human Collagen III protein

Human

COL3A1

Collagen alpha-1(III) chain

Collagen type III occurs in most soft connective tissues along with type I collagen.