Anti-CLEC5A Polyclonal Antibody is a Rabbit antibody targeting CLEC5A. Anti-CLEC5A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Pig,Cow,Horse,Rabbit)

1. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CLEC5A/MDL1 Polyclonal Antibody, Unconjugated (TMAB-00447) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample: Spleen (Mouse) Lysate at 40 μg
Primary: Anti-CLEC5a (TMAB-00447) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
3. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CLEC5A) Polyclonal Antibody, Unconjugated (TMAB-00447) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Sample:
Lane 1: Mouse Spinal cord tissue lysates
Lane 2: Rat Spinal cord tissue lysates
Primary: Anti-CLEC5A (TMAB-00447) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 37 kDa

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: mouse CLEC5A

Mouse

Antiviral Signaling,Macrophage / Inflamm.,Host Virus Interaction